With the birth of first IVF calf of a buffalo breed namely Banni in the country, India’s OPU – IVF work has reached to next level. This first IVF Banni calf is born out of 6 Banni IVF pregnancies established at the doorsteps of a farmer, Vinay L Wala of Sushila Agro Farms, located at Dhanej in Somnath district of Gujarat, Ministry of Fisheries, Animal Husbandry & Dairying, Government of India has said today.
Prime Minister Narendra Modi had talked about the Banni buffalo breed during his visit to Kutch region of Gujarat on December 15, 2020. The very next day, i.e. December 16, 2020, Ovum Pick-Up (OPU) and aspiration processes for the in vitro fertilisation (IVF) of Banni buffaloes were planned.
The scientists aspirated three Banni buffaloes in Sushila Agro Farms. They subjected 29 oocytes (egg cells) to intervaginal culture device (IVC) from these three Banni buffaloes. Total 20 oocytes from one of them were subjected to IVC.
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In fact, 20 oocytes from one donor resulted in 11 embryos. Embryo transfer (ET) was done with nine embryos, which resulted in three IVF pregnancies. Total five oocytes from second donor resulted in five embryos (100 per cent). Of five embryos, four were selected for the ET which resulted in two pregnancies. From the 4 oocytes of the third donor, two embryos were developed and the embryo transfer resulted in one pregnancy.
Overall, 18 embryos were developed from 29 oocytes (62 per cent BL rate). The ET of 15 embryos resulted in six Banni pregnancies (40 per cent pregnancy rate). Of these six pregnancies, the very first IVF Banni calf is born today. This is the first Banni buffalo IVF calf in the country.
Both the government and the scientist fraternity see a great potential in the field of IVF of buffaloes and are striving hard to improve cattle wealth in the country.